ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

ChAT-immunoreactivity (RED) in cholinergic neurons within the mouse caudate nucleus in a paraformaldehyde-fixed (4%) paraffin-embedded section of a transgenic adult male C57Black6 mouse. The green staining is low-level GFP immunoreactivity in this transgenic animal. Dr. Felix Eckenstein, University of Vermont

Anti-Choline Acetyltransferase (ChAT)

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Details

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P28329, NCBI) and mouse (Q03059, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC

Protein Name / Synonyms: Choline O-acetyltransferase (CHOACTase) (ChAT) (Choline acetylase) (EC 2.3.1.6)

Gene ID: CHAT

Antibody Registry ID (RRID): AB_2313537

Physical State: Liquid

Validation and Application Notes

Western Blot Dilution Range: Not Recommended

IHC Dilution Range: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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