Image taken from Gerry Shaw's EnCorBio website.  According to this page: "Mixed neuron-glial cultures stained with CPCA-GAP43 (green) and rabbit antibody to alpha-II spectrin (RPCA-aII-Spec, red), and DNA (blue). The GAP43 antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles."

Image taken from Gerry Shaw's EnCorBio website. According to this page: "Mixed neuron-glial cultures stained with CPCA-GAP43 (green) and rabbit antibody to alpha-II spectrin (RPCA-aII-Spec, red), and DNA (blue). The GAP43 antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles."

GAP-43 immunoreactivity in a neuroblast within a neurosphere culture.  Chicken anti-GAP-43 antibody (1:500 dilution), Fluorescein-goat anti-chicken IgY secondary antibody (Aves Labs, 1:500).  Photomicrograph by Hoda Ilias, Aves Labs.

GAP-43 immunoreactivity in a neuroblast within a neurosphere culture. Chicken anti-GAP-43 antibody (1:500 dilution), Fluorescein-goat anti-chicken IgY secondary antibody (Aves Labs, 1:500). Photomicrograph by Hoda Ilias, Aves Labs.

Image taken from Gerry Shaw's EnCorBio website.  According to this page: "Western blots of extract of cow hippocampus stained with CPCA-GAP43. A prominent band running at ~43 kDa represents GAP43."

Image taken from Gerry Shaw's EnCorBio website. According to this page: "Western blots of extract of cow hippocampus stained with CPCA-GAP43. A prominent band running at ~43 kDa represents GAP43."

Anti-Growth-Associated Protein, 43 kDa (GAP43)

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Product Details


Details

Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of mouse GAP-43 coupled to keyhole limpet hemocyanin. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Volume: 200 µL

Concentration: 2 mg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Neuromodulin (Axonal membrane protein GAP-43) (Growth-associated protein 43) (Neural phosphoprotein B-50) (pp46)

Gene ID: GAP43

Antibody Registry ID (RRID): AB_2313546

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 43 kDa

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:1000 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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