Metabotropic glutamate receptor type 5 (mGluR5, 1:1000 dilution, Aves Labs Cat.# ER5) immunoreactivity (GREEN) and neurofilament-M (NF-M, Rockland, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.
Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the human (NP_001137303.1, NCBI) and mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Volume: 1000 µL
Concentration: 300 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC
Protein Name / Synonyms: Metabotropic glutamate receptor 5 (mGluR5)
Gene ID: GRM5 GPRC1E MGLUR5
Antibody Registry ID (RRID): AB_2313544
Physical State: Liquid
Validation and Application Notes
IHC Dilution Range: 1:1000-1:2000
This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.