(From Dr. Gerry Shaw's EnCor Biotech website).  Rat mixed neuron/glial cultures stained with CPCA-Peri, (green channel) and the EnCor rabbit polyclonal antibody to the neurofilament subunit α-internexin RPCA-a-Int (red channel). These cultures contain mostly neurons which are rich in α-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of both peripherin and α-internexin, the green and r

(From Dr. Gerry Shaw's EnCor Biotech website). Rat mixed neuron/glial cultures stained with CPCA-Peri, (green channel) and the EnCor rabbit polyclonal antibody to the neurofilament subunit α-internexin RPCA-a-Int (red channel). These cultures contain mostly neurons which are rich in α-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of both peripherin and α-internexin, the green and r

(From Dr. Gerry Shaw's EnCor Biotech website).  Rat mixed neuron/glial cultures stained with CPCA-Peri, (green channel) and the EnCor rabbit polyclonal antibody to the neurofilament subunit α-internexin RPCA-a-Int (red channel). These cultures contain mostly neurons which are rich in α-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of both peripherin and α-internexin, the green and r

(From Dr. Gerry Shaw's EnCor Biotech website). Rat mixed neuron/glial cultures stained with CPCA-Peri, (green channel) and the EnCor rabbit polyclonal antibody to the neurofilament subunit α-internexin RPCA-a-Int (red channel). These cultures contain mostly neurons which are rich in α-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of both peripherin and α-internexin, the green and r

(From Dr. Gerry Shaw's EnCor Biotech website).  These are Coomassie Brilliant Blue stained whole protein extracts of adult rat cortex (lane 1), brain stem (lane 2), cerebellum (lane 3), and spinal cord (lane 4) separated on 8% SDS-PAGE. The major neurofilament subunits are indicated by “NF-L”, “NF-M” and ”NF-H”, spectrin/fodrin by “S/F” and Microtubule associated protein 2 by “MAP2”.  Right panel is a western blot of similar preparations in the reverse orientation, where lane 1 is spinal cord, lane 2 is bra

(From Dr. Gerry Shaw's EnCor Biotech website). These are Coomassie Brilliant Blue stained whole protein extracts of adult rat cortex (lane 1), brain stem (lane 2), cerebellum (lane 3), and spinal cord (lane 4) separated on 8% SDS-PAGE. The major neurofilament subunits are indicated by “NF-L”, “NF-M” and ”NF-H”, spectrin/fodrin by “S/F” and Microtubule associated protein 2 by “MAP2”. Right panel is a western blot of similar preparations in the reverse orientation, where lane 1 is spinal cord, lane 2 is bra

Anti-Peripherin (PER)

  • Sale
  • Regular price $245



Product Details | ↓ Citations


Details

Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Volume: 200 µL

Concentration: 2 mg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Peripherin (Neurofilament 4)

Gene ID: PRPH NEF4 PRPH1

Antibody Registry ID (RRID): AB_2313558

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 60 kDa

Western Blot Dilution Range: 1:2000-1:5000

IHC Dilution Range: 1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


back to top


Citations


back to top