Immunocytochemical staining of NF-M immunoreactivity (GREEN) and Glial Fibrillary Acidic Protein (GFAP, RED) in a culture of mouse cortical neurons.  The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.

Immunocytochemical staining of NF-M immunoreactivity (GREEN) and Glial Fibrillary Acidic Protein (GFAP, RED) in a culture of mouse cortical neurons. The chicken anti-NFM immunostaining was performed using a 1:2000 dilution, and the GFAP immunostaining was performed using a 1:5000 dilution. Photomicrographs from Dr. Gerry Shaw, EnCor Biotechnology, Inc.

Anti-NF-M (Neurofilament 160 kDa) (NFM)

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Details

NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Volume: 200 µL

Concentration: 2 mg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Neurofilament medium polypeptide (NF-M) (160 kDa neurofilament protein) (Neurofilament 3) (Neurofilament triplet M protein)

Gene ID: NEFM NEF3 NFM

Antibody Registry ID (RRID): AB_2313554

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 150 kDa

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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